Journal: Biomaterials

Article Title: 3D brown adipogenesis to create “Brown-Fat-in-Microstrands”

doi: 10.1016/j.biomaterials.2015.10.017

Figure Lengend Snippet: Differentiation scheme for CCE ES cells in microstrands timeline (a), determination of optimal brown adipogenesis differentiation of CCE ES cells within alginate hyrogel microstrands in terms of liquid and gel interior (b and c), 100 or 250 µm inner diameter (d and e), and oil red O staining for 3.3 or 8.3 nM BMP 7 (f and g). Complete time course using these conditions is also represented (h–m) along with oil red O staining at day 27 (n). Scale bar = 100 µm.

Article Snippet: The mouse CCE ES cell line was provided by StemCell Technologies, Inc. (Vancouver, Canada) [ 82 , 83 ].

Techniques: Staining

Journal: Biomaterials

Article Title: 3D brown adipogenesis to create “Brown-Fat-in-Microstrands”

doi: 10.1016/j.biomaterials.2015.10.017

Figure Lengend Snippet: qPCR analysis of gene expression of brown adipocyte markers in mouse CCE ESCs grown in 3D alginate hydrogel microstrands compared to untreated control (n=3).

Article Snippet: The mouse CCE ES cell line was provided by StemCell Technologies, Inc. (Vancouver, Canada) [ 82 , 83 ].

Techniques: Gene Expression, Control